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    When it comes to seeing their doctor, many men will figure out a way not to. Men have fewer routine encounters with the medical system as young people. They worry they’ll find out something is wrong and they’re uncomfortable with physical exams, particularly the prostate check. There is one medical problem, however, that tends to send men straight to the doctor: erectile dysfunction (ED). Experts in men’s health say American men who have ED come in seeking prescriptions for one of the three medications available for the condition: Cialis, Levitra, or Viagra. They discover, though, that most health insurers don’t cover the medications, so many men wind up paying as much as $50 to $60 per pill out of pocket for them. That cost has helped create an illegal online market and demand for ED drugs from Canadian pharmacies. ED almost always has an underlying cause relating to a man’s physical or mental health, says David Gremillion, MD, of the University of North Carolina School of Medicine. For the development of multi-omics integrative methodologies we considered necessary to select a system that would allow the generation of both large number of samples and sample material. For this reason, we selected a system, established by the Fisher/Merkenschlager lab (ICL), that models the transition from the , where B cell progenitors undergo growth arrest and differentiation. The approach involves a pre-B cell line, B3, and an inducible version of the Ikaros transcription factor, Ikaros-ERt2. Importantly for STATegra, B3 cells can be grown indefinitely before induction of Ikaros to produce the necessary material for all This model is relevant not only in the context of basic science, but has clinical interest. For instance genetic deletion of Ikaros may result in severe developmental disturbances such as a complete block in B-cell development due to a failure of lymphoid-primed multipotent progenitors to progress toward common lymphoid progenitors. Additionally, IKZF1 (Ikaros) is frequently mutated or deleted in acute lymphoblastic leukemias, especially in cases with B-cell receptor (BCR)-ABL1 translocations, poor clinical outcome, and a high risk of relapse. A preliminary transcription-based study of pre B-CD by the B3 cell line (which resembles pre B-C’, (Brown et al., 1997; Thompson et al., 2007)) was described in (Ferreiros et al., 2013) where a retroviral gene transfer was used to express either wild-type Ikaros (HA-Ikaros-IRES-GFP) or the dominant negative Ikaros DNA-binding mutant 159A (HA-159A Ikaros-IRES-GFP). The positive results shown by (Ferreiros-Vidal et al., 2013) encouraged us in the STATegra Consortia to use it for the development of multi-omic integrative analysis tools. In the case of the wild-type Ikaros the gene is transcribed, the protein is translated but tethered into the cytoplasm until the addition of 4-hydroxy tamoxifen; the process is simplified in the following figure. The control case served to differentiate between the effects of taxomifen and the effects of Ikaros. In STATegra we have generated several omics data types: RNA-seq, mi RNA-seq, RRBS-Seq, DNase-seq, Proteomics and Metabolomics.

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